Members of the Rail, Maritime, and Transport union (RMT) will continue strike action and will walk out on Friday and Saturday evenings.
The strikes will mainly affect the Central and Victoria lines as passengers are warned disruption could last until June.
Union members are striking after the return of the Night Tube which will see drivers cover four-night shifts a year, however, the RMT union does not want the return of the night tube.
However London Underground has assured that there will be no job cuts, the option of permanent work for those on part-time contracts, and only scheduling up to four night shift weekends per year.
Managing director of the London Underground Andy Lord said: "We’re disappointed that once again the RMT is continuing to push for strike action that is likely to cause further unnecessary disruption.
“We’re calling on the RMT to join us for talks so we can work together to resolve this dispute around roster changes, which mean no job losses and greater flexibility for drivers."
Adding that he wanted to apologise to passengers for "the impact this unnecessary action will have on their journeys.”
RMT general secretary Mick Lynch has responded saying that: "If London Underground and the mayor thought this fight for progressive and family-friendly working practices was going away they need to think again.
“RMT has repeatedly put forward cost neutral proposals that would repair the damage unleashed by deleting 200 driver posts and which would dig LU out of this mess."
Lynch also felt they have been ignored and in return, there will be "severe consequences for Londoners through to the summer.”
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